Plasmid Miniprep

Catalog No. 7100-05: DnaUs Plasmid Miniprep, Size: 50 Reactions
Catalog No. 7100-25: DnaUs Plasmid Miniprep, Size: 250 Reactions
Catalog No. 7110-05: DnaUs Plasmid Miniprep High Capacity, Size: 50 Reactions
Catalog No. 7110-25: DnaUs Plasmid Miniprep High Capacity, Size: 250 Reactions
Catalog No. 7150-04: DnaUs 96-Well Plasmid High-Throughput Filter Kit, Size: 4 Reactions
Catalog No. 7150-20: DnaUs 96-Well Plasmid High-Throughput Filter Kit, Size: 20 Reactions

Description

Key to the kit is our proprietary DNA binding systems that allow the high efficient reversible binding of DNA to the ezCapture matrix while proteins and other impurities are removed by wash buffer. Nucleic acids

The plasmid miniprep kit is designed for fast and efficient purification of plasmid DNA from up to 4 ml of E. coli culture. The mini column has a plasmid DNA binding capacity of 50 ug. The yield from 1 ml culture is typically around 8 to 12 ug. The purified DNA is ready for downstream applications such as cloning/subcloning, RFLP, sequencing, and transfection of robust cells such as HEK293 cells.

The plasmid miniprep high capacity kit is designed for fast and efficient purification of plasmid DNA from 1 ml to 15 ml of E. coli culture. The mini column has a plasmid DNA binding capacity of 80 ug. The yield from 1 ml culture is typically around 8 to 12 ug. The purified DNA is ready for downstream applications such as cloning/subcloning, RFLP, sequencing, and transfection of robust cells such as HEK293 cells.

The DnaUs 96-well plasmid kit provides an easy and fast method for isolating high quality plasmid DNA in a high throughput format. The key to this system is ezCapture matrix that avidly, but reversibly, binds DNA under optimized buffer condition while proteins and other unwanted contaminants are removed by wash buffer. The lysate clearance plate obviates the time-consuming centrifugation step and result in increase of DNA recovery up to 20%. By using the 96-well kit, up to 96 samples can be simultaneously processed in less than 90 minutes. The typical DNA yield from 1.5 ml of E. coli culture is around 8 to 12 ug.