DnaUs Hot Start Taq DNA polymerase High Fidelity is a proprietary enzyme mixture of Taq DNA polymerase, proof reading enzyme, and ant-Taq antibodies and is designed for robust amplification of a broad range of targets with high fidelity. It is an antibody-inactivated hot-start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step reaches denaturation temperature (94ºC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The use of this antibody-based hot start enzyme complex allows for convenient room temperature reaction set-up and reduces PCR optimization effort and contamination risk. DnaUs Hot Start Taq DNA polymerase High Fidelity has an error rate more than 6-fold lower than Taq DNA polymerase alone and is ideal for 1 kb to 12 kb target detection, cloning, and sequencing. The enzyme is provided with 10XHF PCR buffer, 50 mM MgSO4 solution, and 10 mM dNTPs solution to perform PCR amplification.
