Glycerol-Free DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step reaches denaturation temperature (94oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The use of this antibody-based hot start enzyme complex allows for convenient room temperature reaction set-up and reduces PCR optimization effort and contamination risk. Glycerol-Free DnaUs Hot Start Taq DNA polymerase has intrinsic 5’->3’ DNA polymerase activity and 5’->3’ exonuclease activity but lacks 3’->5’ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa and displays optimal activity at a temperature between 70-74oC. The enzyme is provided with 10XPCR buffer and 50 mM MgCl2 solution to perform PCR amplification.
