DnaUs Taq DNA polymerase is purified from E.coli. expressing a cloned Thurmus aquaticus DNA polymerase gene. This enzyme has an intrinsic 5’->3’ DNA polymerase and a 5’->3’ exonuclease activity but lacks a 3’->5’ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa and displays optimal activity at temperature between 70-74ºC. The enzyme is provided with 10 mM dNTPs, 10XPCR buffer and 50 mM MgCl2 solution to perform PCR amplification.
