LeGene Express 1st Strand cDNA Synthesis System, 200 reactions

Protocols and Manuals

Description

LeGene Express 1st Strand cDNA Synthesis System is optimized to synthesize high yield cDNA from total or poly( A)+ purified RNA. The system can be used with a broad range of RNA template concentrations, from as low as 10 pg to as high as to 5 µg of total RNA. LeGene Express 1st Strand cDNA Synthesis reverse transcriptase is a mixture of RnaUs Script reverse transcriptase, an engineered MMLV RT and a ribonuclease inhibitor protein. A wide range of RNA targets from 100 bp to 10 kb can be detected with this system.

It is ideally suited for gene isolation, high throughput expression profile tudies using real-time quantitation, and end-point RT-PCR. The system contains all the components needed for first-strand cDNA synthesis. The reagents are sufficient for 50 or 200 cDNA synthesis reactions of 20 µl reaction volume.