LeGene Premium One-Step RT-PCR is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube. The proprietary 2X Reaction Mix contains optimized buffer including dNTPs, Mg2+, enhancer, and stabilizer. This system uses a mixture of an engineered RnaUsScript RT and DnaUs Hot Start Taq DNA polymerase in an optimized reaction buffer and can detect RNA targets up to 4 kb. DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. Once the PCR step reaches denaturation temperature (94oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The amount of input total RNA can range from 1 pg to 2 μg. Sufficient reagents are provided for 250 amplification reactions of 50 μl each.