LeGene 1-Step RT-PCR Master Mix Sky Blue is a ready-to-use and ready-to-load 2X concentrated master mix that contains all necessary components for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific targets up to 2.5 kb from either total RNA or mRNA in a single tube. The proprietary 2X Reaction Master Mix contains an optimized buffer with RnaUsScript-20 reverse transcriptase, DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+,stabilizers, and blue tracking dye. The amount of input total RNA can range from 1 pg to 1 μg. Sufficient reagents are provided for 200 amplification reactions of 50 μl reaction volume each.