Fr-Fr 1-Step qRT-PCR Master Mix, ROX (5X), 2,500 X 20μl reactions

Protocols and Manuals

Description

Fr-Fr 1-Step qRT-PCR Master Mix, ROX is a 5X concentrated formulation mix. It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments. The proprietary 5X qRT-PCR Master Mix, ROX contains a fusion Reverse Transcriptase –RnaUsScript-20 RT, DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, stabilizer and an optimal concentration of ROX for instruments that require ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system. DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. RnaUsScript-20 RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The system enables highly sensitive detection from as few as 1 copy of a target gene, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA. Sufficient reagents are provided for 2,500 amplification reactions of 20 μl each.