ViralPath1-Tube RT-qPCR SuperMix, ROX (2X), 400X 20μl reactions

Protocols and Manuals

Description

ViralPath 1-Tube RT-qPCR SuperMix, ROX is designed for robust and reliable one-step pathogenic virus detection as well as gene expression workflows It is a 2X concentrated formulation master mix and designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA in a single tube for the detection and quantification of RNA using real-time detection instruments. The proprietary 2X RT-qPCR SuperMix contains thermostable RT, thermostable Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, stabilizers and an optimal concentration of ROX for instruments that require ROX for a reference signal. Please consult our website and Instrument compatibility section below for the correct product for your real-time PCR system. The system enables highly sensitive detection from as few copies of a target gene (<10) with a broad dynamic range that supports accurate quantification. Sufficient reagents are provided for 100, 400, 1,000, and 2,500 amplification reactions of 20 μl each.