Fr-Fr SG 1-Step qRT-PCR Master Mix is a 5X concentrated formulation mix. It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments. The proprietary 5X SG qRT-PCR Master Mix contains a fusion Reverse Transcriptase –RnaUsScript-20 RT, DnaUs Hot Start Taq DNA polymerase, SG Dye, dNTPs, Mg2+, enhancer, and stabilizer. Fr-Fr SG 1-Step qRT-PCR Master Mix does not contain an internal reference dye. Please consult our website and Instrument compatibility section below for the correct product for your real-time PCR system. DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. RnaUsScript-20 RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The system enables highly sensitive detection from as few as 1 copy of a target gene, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA. Sufficient reagents are provided for 125 amplification reactions of 20 μl each.