COVID-19 One-Step RT-qPCR System Low ROX, 500 X 20μl reactions

Protocols and Manuals

Description

COVID-19 One-Step RT-qPCR System, Low ROX is consist of Enzyme Mix and 2X Reaction Mix, It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments. The proprietary Enzyme Mix contains an engineered RnaUsScript Reverse Transcriptase (RNaseH minus), DnaUs Hot Start Taq DNA polymerase, stabilizers. 2X Reaction Mix consists of a proprietary buffer system, 6 mM MgCl2, dNTPs, enhancers and stabilizers and an optimal concentration of ROX for instruments that require low ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system. DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. RnaUsScript RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The system enables highly sensitive detection from as few as 10 copies of SARS-CoV-2 target genes (N1 and N2), with a broad dynamic range that supports accurate quantification of mRNA copies. Sufficient reagents are provided for 500 amplification reactions of 20 μl each.