RnaUsScript-Gold Reverse Transcriptase, 20,000 units (200,000 units/ml)

Protocols and Manuals

Catalog No. 6159-20: RnaUsScript-Gold Reverse Transcriptase, Size: 20,000 U

Description

RnaUsScript-Gold Reverse Transcriptase (RNaseH Minus) is an engineered from Moloney Murine Leukemia Virus RT to eliminate RNaseH activity and enhance cDNA yield at an elevated temperature up to 60 oC..RnaUsScript-Gold RT is a single polypeptide chain with a molecular weight of approximately 78.2 kDa, is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA strand from a single-stranded RNA template to which a primer has been annealed (1,2). RnaUsScript-Gold RT also efficiently extend primers hybridized to single-stranded DNA. This enzyme is expressed in E. coli (2) and purified to free of exogenous RNase activity, a common contaminant in commercial preparations of reverse transcriptase. The enzyme is used to synthesize first-strand cDNA up to 13 kb and 5X cDNA Synthesis buffer is provided.