LeGene RnaUs Total Bacterial RNA Prep provides an easy and fast method for isolating total RNA from Gram-positive (B. subtilis) or Gram-negative (E. coli) Bacteria within 30 min. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including RT-PCR, real time RT-PCR, Northern blotting, RNase protection and primer extension, miRNA cloning and amplification, and Next Generation Sequencing.